INTRODUCTION TO ENDOSPORE STAINING Some bacteria are capable of changing into dormant structures that are metabolically…
Category: Microbiology Practicals
PREPARATION OF BLOOD AGAR MEDIUM USING TRYPTICASE SOY AGAR BASE
The most widely used enriched medium for the growth of Fastidious organisms (those microbes that requires…
PREPARATION OF BLOOD AGAR MEDIUM FROM NUTRIENT AGAR BASE
The most widely used enriched medium for the growth of Fastidious organisms (those microbes that requires…
CAPSULE STAINING TECHNIQUE – INTRODUCTION, METHODS, PRINCIPLE, PROCEDURE & RESULTS
INTRODUCTION TO CAPSULE STAINING TECHNIQUE A capsule in bacteria is the result of amorphous viscid secretion…
NEGATIVE STAINING – INTRODUCTION, PRINCIPLE, PROCEDURE, RESULTS & APPLICATIONS
INTRODUCTION TO NEGATIVE STAINING TECHNIQUE Sometimes it is more convenient to determine the overall bacterial cell…
EXAMINATION OF SPUTUM SPECIMEN IN MICROBIOLOGY LABORATORY
The term “Sputum” often used interchangeably with the “phlegm” but technically if we take a look…
INTERPRETATION OF RESULTS & REPORTING OF AFB (ACID FAST BACILLI) IN SPUTUM SMEARS
Acid-fast staining is the most commonly used staining technique for the identification of Mycobacterium species especially…
PREPARATION OF MACCONKEY BROTH MEDIUM IN LABORATORY
The MacConkey Broth medium, which is similar to the first ever developed solid differential medium for…
PREPARATION OF TRYPTICASE SOY BROTH MEDIUM (TSB) a.k.a TRYPTIC SOYA BROTH OR TRYPTONE SOYA BROTH IN LABORATORY
In Microbiology laboratory, Bacteria need to be cultured in the for various purposes like identification of…
COMPOSITION OF MACCONKEY AGAR MEDIUM (MAC MEDIUM) & MODIFICATIONS AS APPLICABLE
The MacConkey Agar Medium commonly called as MAC medium is first artificial differential solid medium prepared…
PREPARATION OF TRYPTICASE SOY AGAR MEDIUM (TSA) a.k.a TRYPTIC SOYA AGAR OR TRYPTONE SOYA AGAR IN LABORATORY
Bacteria need to be cultured in the laboratory for various purposes like identification of the causative…
PREPARATION OF SABOURAUD DEXTROSE BROTH MEDIUM (SABOURAUD LIQUID MEDIUM) FOR FUNGUS CULTURE IN LABORATORY
Fungi are needed to be cultured in the laboratory for various purposes like identification of the…
PREPARATION OF MACCONKEY AGAR MEDIUM (MAC) IN LABORATORY
The first ever developed solid differential medium for the growth of bacteria on the globe was…
PREPARATION OF SABOURAUD DEXTROSE AGAR (SDA) MEDIUM IN LABORATORY
Fungi are needed to be cultured in the laboratory for various purposes like identification of the…
PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY
The McFarland Standards are commonly used in Antibiotic Susceptibility Test (AST) to standardize the approximate number…
Gram Staining – Principle, Requirements, Procedure & Interpretation
WHAT IS GRAM STAINING TECHNIQUE? Gram staining is the most important and widely used differential staining…
PREPARATION OF MUELLER HINTON AGAR MEDIA (MHA) IN LABORATORY
The well-known solid media for the Antibiotic Sensitivity Test a.k.a. Antibiotic Susceptibility Test was developed by…
PREPARATION OF ACID FAST REAGENTS IN LABORATORY
PREPARATION OF ACID FAST REAGENTS The Acid-Fast staining technique was initially developed in 1882 by Paul…
HANGING DROP PREPARATION TO EXAMINE THE MOTILITY OF MICROORGANISM / BACTERIA
Hanging Drop Preparation is the useful technique employed in the laboratory for the microscopic examination of…
STREAK PLATE CULTURE TECHNIQUE FOR THE ISOLATION OF MICROORGANISM / BACTERIA IN PURE CULTURE
A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from…