MICROBIOLOGY PRACTICALS | Paramedics World

ENDOSPORE STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO ENDOSPORE STAINING Some bacteria are capable of changing into dormant structures that are metabolically…

PREPARATION OF BLOOD AGAR MEDIUM USING TRYPTICASE SOY AGAR BASE

The most widely used enriched medium for the growth of Fastidious organisms (those microbes that requires…

PREPARATION OF BLOOD AGAR MEDIUM FROM NUTRIENT AGAR BASE

The most widely used enriched medium for the growth of Fastidious organisms (those microbes that requires…

CAPSULE STAINING TECHNIQUE – INTRODUCTION, METHODS, PRINCIPLE, PROCEDURE & RESULTS

INTRODUCTION TO CAPSULE STAINING TECHNIQUE A capsule in bacteria is the result of amorphous viscid secretion…

NEGATIVE STAINING – INTRODUCTION, PRINCIPLE, PROCEDURE, RESULTS & APPLICATIONS

INTRODUCTION TO NEGATIVE STAINING TECHNIQUE Sometimes it is more convenient to determine the overall bacterial cell…

EXAMINATION OF SPUTUM SPECIMEN IN MICROBIOLOGY LABORATORY

The term “Sputum” often used interchangeably with the “phlegm” but technically if we take a look…

INTERPRETATION OF RESULTS & REPORTING OF AFB (ACID FAST BACILLI) IN SPUTUM SMEARS

Acid-fast staining is the most commonly used staining technique for the identification of Mycobacterium species especially…

PREPARATION OF MACCONKEY BROTH MEDIUM IN LABORATORY

The MacConkey Broth medium, which is similar to the first ever developed solid differential medium for…

PREPARATION OF TRYPTICASE SOY BROTH MEDIUM (TSB) a.k.a TRYPTIC SOYA BROTH OR TRYPTONE SOYA BROTH IN LABORATORY

In Microbiology laboratory, Bacteria need to be cultured in the for various purposes like identification of…

COMPOSITION OF MACCONKEY AGAR MEDIUM (MAC MEDIUM) & MODIFICATIONS AS APPLICABLE

The MacConkey Agar Medium commonly called as MAC medium is first artificial differential solid medium prepared…

PREPARATION OF TRYPTICASE SOY AGAR MEDIUM (TSA) a.k.a TRYPTIC SOYA AGAR OR TRYPTONE SOYA AGAR IN LABORATORY

Bacteria need to be cultured in the laboratory for various purposes like identification of the causative…

PREPARATION OF SABOURAUD DEXTROSE BROTH MEDIUM (SABOURAUD LIQUID MEDIUM) FOR FUNGUS CULTURE IN LABORATORY

Fungi are needed to be cultured in the laboratory for various purposes like identification of the…

PREPARATION OF MACCONKEY AGAR MEDIUM (MAC) IN LABORATORY

The first ever developed solid differential medium for the growth of bacteria on the globe was…

PREPARATION OF SABOURAUD DEXTROSE AGAR (SDA) MEDIUM IN LABORATORY

Fungi are needed to be cultured in the laboratory for various purposes like identification of the…

PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY

The McFarland Standards are commonly used in Antibiotic Susceptibility Test (AST) to standardize the approximate number…

GRAM’s STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO GRAM STAINING Grams staining technique is the most important and widely used differential staining…

PREPARATION OF MUELLER HINTON AGAR MEDIA (MHA) IN LABORATORY

The well-known solid media for the Antibiotic Sensitivity Test a.k.a. Antibiotic Susceptibility Test was developed by…

PREPARATION OF ACID FAST REAGENTS IN LABORATORY

PREPARATION OF ACID FAST REAGENTS The Acid-Fast staining technique was initially developed in 1882 by Paul…

HANGING DROP PREPARATION TO EXAMINE THE MOTILITY OF MICROORGANISM / BACTERIA

Hanging Drop Preparation is the useful technique employed in the laboratory for the microscopic examination of…

STREAK PLATE CULTURE TECHNIQUE FOR THE ISOLATION OF MICROORGANISM / BACTERIA IN PURE CULTURE

A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from…