MICROBIOLOGY PRACTICALS | PARAMEDICS WORLD

MICROBIOLOGY PRACTICALS

GRAM STAINING

GRAM’s STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO GRAM STAINING Grams staining technique is the most important and widely used differential staining technique in Bacteriology. This technique was developed in 1884 by a Danish bacteriologist, Hans Christian Gram. The Gram staining technique differentiates the mixed culture cells into two terms – One which retains the color of primary stain is known as Gram-Positive Bacteria and the cells which get decolorized and takes the counterstain are known ...
Continue Reading.....
PREPARATION OF ACID FAST REAGENTS ACID FAST STAINING PROCEDURE, INTERPRETATION

ACID FAST STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE, INTERPRETATION

INTRODUCTION TO ACID FAST STAINING Acid fast staining technique was developed in 1882 by Paul Ehrlich and modified by Ziehl & Nielsen in 1890 hence called Z.N. Staining or Ziehl-Neelsen staining. Certain bacteria and Actinomycetes have many components in the cell wall and their cell wall has little permeability. Majority of bacteria can be stained with simple stain but the number of Mycobacteria and Nocardia can only be stained by ...
Continue Reading.....
ENDOSPORE STAINING

ENDOSPORE STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO ENDOSPORE STAINING Some bacteria are capable of changing into dormant structures that are metabolically inactive and do not grow or reproduce. Since these structures are formed inside the cells, hence called endospores. These are remarkably resistant to heat, radiation, chemicals and other agents, that are typically lethal to the organisms. The heat resistance of the spores has been linked to their high content of calcium and dipicolinic acid ...
Continue Reading.....
ALBERT STAINING - CORYNEBACTERIUM DIPTHERIAE

ALBERT STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & RESULT INTERPRETATION

INTRODUCTION TO ALBERT STAINING Corynebacterium is the genus of Gram +ve, non-acid-fast, non-sporing, non-motile bacilli. The most important member of the genus is Corynebacterium diphtheriae, the causative agent of Diphtheria disease in children. The C. diptheriae or diphtheria bacillus was first described by Klebs but Loffler was the first to cultivate it in the laboratory and also called as the Klebs-Loffler’s bacillus (KLB) They form metachromatic granules, these granules are ...
Continue Reading.....
negative staining technique - negative staining method - principle - procedure - results - application

NEGATIVE STAINING – INTRODUCTION, PRINCIPLE, PROCEDURE, RESULTS & APPLICATIONS

INTRODUCTION TO NEGATIVE STAINING TECHNIQUE Sometimes it is more convenient to determine the overall bacterial cell morphology without any use of harsh staining or heat fixing techniques that changes the shape of the bacterial cells. The negative staining is preferred in cases when the bacterium does not stain well (e.g. some of the spirochetes) or when it is desirable to confirm observations made on the shape and size or Morphology ...
Continue Reading.....
bacterial smear preparation - bacterial smear - microscopic examination of specimen in microbiology - bacterial smear preparation in lab - how to prepare a bacterial smear - Who discovered bacteria

PREPARATION OF AN IDEAL BACTERIAL SMEAR

A bacterial smear is required particularly for the microscopic examination of the specimen or the culture for the identification of bacteria which is done by preparing the smear from the specimen or culture followed by staining with the appropriate stains, e.g. Gram staining. The purpose of making the smear is to fix the specimen or the bacterial cells from the culture onto the microscopic glass slide so that it will ...
Continue Reading.....
hanging drop preparation - hanging drop method - how to check the motility of bacteria - what is hanging drop method - what is the use of hanging drop - how to prepare hanging drop -

HANGING DROP PREPARATION TO EXAMINE THE MOTILITY OF MICROORGANISM / BACTERIA

Hanging Drop Preparation is the useful technique employed in the laboratory for the microscopic examination of living microorganisms (viable microorganisms), especially the bacteria without staining them and to see their motility due to flagella. Flagella is the organ of locomotion in Bacteria and various types of arrangement of Flagella is found in the bacteria which causes the variation in motility of bacteria as some a highly motile and some are ...
Continue Reading.....
pour plate culture technique - culture techniques in microbiology - culture methods in microbiology - pour plate culture method - simple culture method - how to culture in microbiology

POUR PLATE CULTURE TECHNIQUE FOR THE ISOLATION OF MICROORGANISM / BACTERIA IN PURE CULTURE

A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures and pour-plate technique is among the simplest technique of isolating the bacteria. The Culture techniques are commonly used in the laboratory for various purposes for which they are intended. The indications for the culture of the organism is mainly done – To demonstrate the cultural characteristics of ...
Continue Reading.....
spread plate culture technique - spread plate method - culture methods in microbiology lab - methods used for the culture of specimen - microbiology culture techniques

SPREAD PLATE CULTURE TECHNIQUE FOR THE ISOLATION OF MICROORGANISM / BACTERIA IN PURE CULTURE

A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures and Spread plate culture technique is among the most widely used culture technique for isolating the bacteria. The Culture techniques are commonly used in the laboratory for various purposes for which they are intended. The indications for the culture of the organism is mainly done – To ...
Continue Reading.....
streak plate culture method - streak plate culture technique - streaking on blood agar - various method of streaking

STREAK PLATE CULTURE TECHNIQUE FOR THE ISOLATION OF MICROORGANISM / BACTERIA IN PURE CULTURE

A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the cultures and Streak plate technique is among the most widely used technique to obtain discrete & Well developed colonies of microbes. The Culture techniques are commonly used in the laboratory for various purposes for which they are intended. The indications for the culture of the organism is mainly done ...
Continue Reading.....
Loading...