PREPARATION OF BLOOD AGAR MEDIUM USING TRYPTICASE SOY AGAR BASE
The most widely used enriched medium for the growth of Fastidious organisms (those microbes that requires some extra nutrients and only grow in enriched medium and not on an ordinary medium are known as fastidious microbes) is one n only our Blood Agar Medium (BAM). The Blood Agar Medium is the simplest yet powerful enriched medium that supports the growth of almost all the microbes and extremely easy to prepare ...
PREPARATION OF BLOOD AGAR MEDIUM FROM NUTRIENT AGAR BASE
The most widely used enriched medium for the growth of Fastidious organisms (those microbes that requires some extra nutrients and only grow in enriched medium and not on an ordinary medium are known as fastidious microbes) is one n only our Blood Agar Medium (BAM). The Blood which is the key component of BAM contains a few inhibitors for certain bacteria such as Hemophilus and Neisseria species and the BAM required to ...
CAPSULE STAINING TECHNIQUE – INTRODUCTION, METHODS, PRINCIPLE, PROCEDURE & RESULTS
INTRODUCTION TO CAPSULE STAINING TECHNIQUE A capsule in bacteria is the result of amorphous viscid secretion released by the bacteria. When this secretion remains loose and un-demarcated it is called a slime layer and when it is organized into a sharply defined structure it is called as Capsule. Most of the capsules are made up of polysaccharides but there are many which are composed of Polypeptides (Proteins). Capsules are usually ...
INTERPRETATION OF RESULTS & REPORTING OF AFB (ACID FAST BACILLI) IN SPUTUM SMEARS
Acid-fast staining is the most commonly used staining technique for the identification of Mycobacterium species especially the Mycobacterium tuberculosis in the sputum samples. After making an Acid-fast stained smear of the specimen a Laboratory technologist has to carefully observe under the microscope for the Red-colored Thin, Beaded curved rods which is a characteristic morphology of Mycobacterium tuberculosis and thereafter counting the no. of bacteria observed per High Power field (HPF) ...
EXAMINATION OF SPUTUM SPECIMEN IN MICROBIOLOGY LABORATORY
The term “Sputum” often used interchangeably with the “phlegm” but technically if we take a look at these words, they have a little bit of difference as…. the mucus secreted from the Respiratory tract is known as the “Phlegm” whereas When Phlegm is mixed with the Saliva it is termed as “Sputum”. The Microbiological examination of Sputum is commonly done as sputum is desired in cases of suspected pulmonary tuberculosis ...
PREPARATION OF MACCONKEY BROTH MEDIUM IN LABORATORY
The MacConkey Broth medium, which is similar to the first ever developed solid differential medium for the growth of bacteria i.e. the MacConkey Agar Medium, formulated by and is named after a British bacteriologist, Alfred Theodore MacConkey in the 20th century, contains almost same components as MAC medium except for Solidifying agent that is Agar-Agar. Besides being a Differential solid medium, MacConkey Broth Medium is a selective and differential medium too ...
PREPARATION OF TRYPTICASE SOY BROTH MEDIUM (TSB) a.k.a TRYPTIC SOYA BROTH OR TRYPTONE SOYA BROTH IN LABORATORY
In Microbiology laboratory, Bacteria need to be cultured in the for various purposes like identification of the causative agent associated with the pathology, for research purposes, preparations of various vaccines as well as for the productions of certain proteins and enzymes, etc. The universal culture medium used in the microbiology laboratories that supports the cultivations of almost all types of bacteria is the Trypticase Soy Agar (TSA) and the Trypticase ...
PREPARATION OF TRYPTICASE SOY AGAR MEDIUM (TSA) a.k.a TRYPTIC SOYA AGAR OR TRYPTONE SOYA AGAR IN LABORATORY
Bacteria need to be cultured in the laboratory for various purposes like identification of the causative agent associated with the pathology, for research purposes, preparations of various vaccines as well as for the productions of certain proteins and enzymes, etc. The universal culture medium used in the microbiology laboratories that supports the cultivations of almost all types of bacteria is the Trypticase Soy Agar Medium (TSA), also known as the ...
COMPOSITION OF MACCONKEY AGAR MEDIUM (MAC MEDIUM) & MODIFICATIONS AS APPLICABLE
The MacConkey Agar Medium commonly called as MAC medium is first artificial differential solid medium prepared in 20th Century by a British Bacteriologist, Alfred Theodore MacConkey after which it was named as MacConkey Agar Medium. MAC medium is a selective medium for Gram-negative bacteria as well as a differential media that different bacteria as Lactose and Non-Lactose fermentors and moreover MAC medium is an Indicator medium that shows two colors ...
PREPARATION OF MACCONKEY AGAR MEDIUM (MAC) IN LABORATORY
The first ever developed solid differential medium for the growth of bacteria on the globe was MacConkey Agar Medium (MAC medium), was formulated by and is named after a British bacteriologist, Alfred Theodore MacConkey in the 20th century. Besides being a Differential solid medium, MacConkey Agar Medium is a selective and Indicator medium too and primarily used for the isolation of Gram-negative and enteric bacilli, the rod-shaped bacteria. Moreover, MAC is the ...
