HISTOPATHOLOGY AND CYTOPATHOLOGY NOTES

MICROTOMY – THE ART OF SECTION CUTTING Microtomy or section cutting is the technique of making the very thin slices of tissue specimens for the microscopic examination to identify the abnormalities or atypical appearance in the tissue (if present) and also for the study of various components of the cells or tissues like Lipids, Enzymes, Antigens or Antibodies (Immunohistochemistry), Cell organelles etc. Before sections are made the tissues require to be embedded in some fluid, which will permeate ...

INTRODUCTION TO MICROTOMES Microtome is an instrument with the help of which sections of tissues are cut and the process of cutting thin sections is known as Microtomy. The thickness of sections produced during microtomy may be between fractions of 50-100 nm, in ultramicrotomy, to several 100 microns. The common range is between 5-10m but both the maximum and minimum thickness is limited by the consistency of relation of the ...

Histological techniques are the techniques which have been developed for the processing of the specimens, mainly tissues, for the proper diagnosis of the diseases associated. The small pieces of the tissues or sometimes whole organs are submitted to the histopathology laboratory for the diagnosis of any abnormalities if present. These tissues or organs need the treatment of certain chemicals to prevent any deterioration or alterations before the exact examination under the ...

INTRODUCTION TO HISTOPATHOLOGY & CYTOPATHOLOGY Cells are the simplest unit of living matter that can maintain life and reproduce within themselves. Groups of these cells unite to perform a specific function, called tissues. The tissues of the body consist of a large number of cells and they are classified according to the shape size and functions of the cells. Histology is the study of typical tissues particularly involving the investigations ...