BIOCHEMISTRY PRACTICALS

A solution is a homogeneous mixture composed of only one phase. In such a mixture, a solute is a substance dissolved in another substance called as the solvent. The concentration of a solute in a solution is a measure of how much of that solute is dissolved in a solvent. Standard solutions are those in which the exact amount of a substance presents in a definite volume of solution. To ...

PREPARATION OF PHOSPHATE BUFFER SOLUTION (pH 5.8 to 7.4) IN LABORATORY A Buffer solution is a special type of solution which helps to maintain a stable pH level when a small amount of acid or alkali is introduced into a solution. A phosphate buffer solution is one of the commonly used buffer solution in biological laboratories and commonly used in Hematology laboratory for diluting the stains. Chemically, the phosphoric acid ...

A spectrophotometer is an instrument used to measure absorbance at various wavelengths. It is similar to calorimeter except that it uses prism or diffraction grating to produce monochromatic light. It can be operated in UV (Ultraviolet) region, Visible spectrum as well as IR (Infrared) region of the electromagnetic spectrum. ⇒ Absorption of light - Light falling on a colored solution is either absorbed or transmitted. A colored solution absorbs all the ...

⇒ A Colorimeter involves the measurement of Color and is the widely used method for finding the concentration of biochemical compounds. It Measures absorbance and wavelength between 400 to 700 nm (nanometer) i.e. from the visible spectrum of light of the electromagnetic spectrum. ⇒ Absorption of light - Light falling on a colored solution is either absorbed or transmitted. A colored solution absorbs all the colors of white light and selectively transmits ...

A solution is a homogeneous mixture composed of only one phase. In such a mixture, a solute is a substance dissolved in another substance called as the solvent. The concentration of a solute in a solution is a measure of how much of that solute is dissolved in a solvent. Standard solutions are those in which the exact amount of a substance presents in a definite volume of solution. To ...

Normal Saline Solution (NSS) is commonly used in various laboratory procedures like in the preparation of Red cells Suspension for the crossmatch, for preparing dilutions of Reagents, for stool examinations, to make the dilutions in serological tests, diagnostic tests etc. There are commercially prepared Normal saline solution available in the market but it can easily be prepared manually in the laboratory whenever required. The normal saline solution is simply the ...

MICROPIPETTES..... Micropipettes are the type of air displacement pipettes that are used to transfer the small quantities of solutions measured in microliters, 1000 µl (microliters) equals to 1 ml (milliliter) and is the maximum amount that is commonly dispensed with the micropipettes. Micropipettes are of two types, the Fixed micropipettes, and the Variable Micropipettes. The Fixed micropipettes are designed to transfer the fixed amount of volume of the liquid. The ...