Biochemical Tests for Klebsiella pneumoniae | Bacteriology Notes

BIOCHEMICAL TESTS FOR KLEBSIELLA PNEUMONIAE (K. PNEUMONIAE)

In this Article, we’re gonna discuss so many biochemical reactions for the well known causative agent of Nosocomial Infections specially Pneumonia, UTI (Urinary Tract Infections), Septicemia, Pyogenic Infections and Sometimes Diarrhea i.e. the Klebsiella pneumoniae abbreviated as K. pneumoniae.

However, only a few reactions are most commonly used and are medically important for distinguishing pathogenic strains of K. pneumoniae from other non- pathogenic strains as well as from other species of Klebsiella, which are as follows:



Indole Negative

Citrate Positive

Urease Positive

Methyl red (MR) Negative

Voges – Proskauer (VP) Positive

Pink colored – LF colonies on MacConkey agar medium.

klebsiella pneumoniae on macconkey agar medium - klebsiella on macconkey agar medium - k. pneumoniae on macconkey agar medium
Klebsiella pneumoniae Growth on MacConkey Agar Medium

Purple colonies on EMB Agar medium without Green Metallic sheen.

Klebsiella pneumoniae on EMB medium - klebsiella pneumoniae on eosin methylene blue medium
Klebsiella pneumoniae growth on EMB medium

Check out the Biochemical tests for Escherichia coli (E.coli)

Besides that of various biochemical reactions and Sugar fermentation tests, certain enzymatic reactions are also medically important to distinguish Klebsiella pneumoniae from other Klebsiella sp.

biochemical tests for klebsiella pneumoniae - citrate positive - vp positive - voges poskauer positive
Klebsiella pneumoniae is Citrate & Voges-Proskauer (VP) Positive

Below is the list of these Enzymatic Reactions and various other biochemical tests for Klebsiella pneumoniae which have great importance in research and for knowledge but are not routinely employed:

TESTSRESULTS
Catalase+VE (POSITIVE)
Citrate+VE (POSITIVE)
Esculinase+VE (POSITIVE)
Indole-VE (NEGATIVE)
Nitrate Reduction+VE (POSITIVE)
ONPG+VE (POSITIVE)
Urease+VE (POSITIVE)
VP (Voges Proskauer)+VE (POSITIVE)
Coagulase-VE (NEGATIVE)
DNase-VE (NEGATIVE)
H2S Production-VE (NEGATIVE)
Methyl Red-VE (NEGATIVE)
Oxidase-VE (NEGATIVE)
Arginine Dehydrolase-VE (NEGATIVE)
Ornithine decarboxylase-VE (NEGATIVE)
Tryptophanase-VE (NEGATIVE)




Klebsiella pneumoniae and other species of Klebsiella ferment various sugars producing acid with gas.

But these sugar fermentation tests are of no diagnostic value in routine laboratory tests except Glucose fermentation test which is commonly used to differentiate Klebsiella pneumoniae, which produces Acid with Gas, from other Pathogenic & non-pathogenic Klebsiella species especially from Klebsiella rhinoscleromatis, which do not produce gas during Glucose fermentation.

The other sugar fermentation tests which are described below are of great importance in research work.

SUGARSRESULTS
Adonitol+VE (POSITIVE)
Arabinose+VE (POSITIVE)
Cellobiose+VE (POSITIVE)
Dextrose+VE (POSITIVE)
Fructose+VE (POSITIVE)
Galactose+VE (POSITIVE)
Glucose+VE (POSITIVE)
Lactose+VE (POSITIVE)
Maltose+VE (POSITIVE)
Mannitol+VE (POSITIVE)
Mannose+VE (POSITIVE)
Melibiose+VE (POSITIVE)
Raffinose+VE (POSITIVE)
Rhamnose+VE (POSITIVE)
Salicin+VE (POSITIVE)
Sorbitol+VE (POSITIVE)
Sucrose+VE (POSITIVE)
Trehalose+VE (POSITIVE)
Xylose+VE (POSITIVE)
Dulcitol-VE (NEGATIVE)

That’s all about the Biochemical tests for Klebsiella pneumoniae

Further Reading:

Differentiation of Escherichia coli from coliforms – Merck

Klebsiella pneumoniae subsp. pneumoniae – VetBact



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