MICROBIOLOGY PRACTICALS | PARAMEDICS WORLD

MICROBIOLOGY PRACTICALS

GRAMS STAINING

GRAM’s STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO GRAM STAINING Grams staining technique is the most important and widely used differential staining technique in Bacteriology. This technique was developed in 1884 by a Danish bacteriologist, Hans Christian Gram. The Gram staining technique differentiates the mixed culture cells into two terms – One which retains the color ...
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ACID FAST STAINING PROCEDURE, INTERPRETATION

ACID FAST STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE, INTERPRETATION

INTRODUCTION TO ACID FAST STAINING Acid fast staining technique was developed in 1882 by Paul Ehrlich and modified by Ziehl & Nielsen in 1890 hence called Z.N. Staining or Ziehl-Neelsen staining. Certain bacteria and Actinomycetes have many components in the cell wall and their cell wall has little permeability. Majority ...
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ENDOSPORE STAINING

ENDOSPORE STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO ENDOSPORE STAINING Some bacteria are capable of changing into dormant structures that are metabolically inactive and do not grow or reproduce. Since these structures are formed inside the cells, hence called endospores. These are remarkably resistant to heat, radiation, chemicals and other agents, that are typically lethal to ...
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ALBERT STAINING - CORYNEBACTERIUM DIPTHERIAE

ALBERT STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO ALBERT STAINING Corynebacterium is the genus of Gram +ve, non-acid-fast, non-sporing, non-motile bacilli. The most important member of the genus is Corynebacterium diphtheriae, the causative agent of Diphtheria disease in children. The C. diptheriae or diphtheria bacillus was first described by Klebs but Loffler was the first to ...
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