MICROBIOLOGY PRACTICALS | PARAMEDICS WORLD

MICROBIOLOGY PRACTICALS

GRAM STAINING

GRAM’s STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION 4.8/5 (5)

INTRODUCTION TO GRAM STAINING Grams staining technique is the most important and widely used differential staining technique in Bacteriology. This technique was developed in 1884 by a Danish bacteriologist, Hans Christian Gram. The Gram staining technique differentiates the mixed culture cells into two terms – One which retains the color of primary stain is known as Gram-Positive Bacteria and the cells which get decolorized and takes the counterstain are known ...
Continue Reading.....
PREPARATION OF ACID FAST REAGENTS ACID FAST STAINING PROCEDURE, INTERPRETATION

ACID FAST STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE, INTERPRETATION

INTRODUCTION TO ACID FAST STAINING Acid fast staining technique was developed in 1882 by Paul Ehrlich and modified by Ziehl & Nielsen in 1890 hence called Z.N. Staining or Ziehl-Neelsen staining. Certain bacteria and Actinomycetes have many components in the cell wall and their cell wall has little permeability. Majority of bacteria can be stained with simple stain but the number of Mycobacteria and Nocardia can only be stained by ...
Continue Reading.....
ENDOSPORE STAINING

ENDOSPORE STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & INTERPRETATION

INTRODUCTION TO ENDOSPORE STAINING Some bacteria are capable of changing into dormant structures that are metabolically inactive and do not grow or reproduce. Since these structures are formed inside the cells, hence called endospores. These are remarkably resistant to heat, radiation, chemicals and other agents, that are typically lethal to the organisms. The heat resistance of the spores has been linked to their high content of calcium and dipicolinic acid ...
Continue Reading.....
ALBERT STAINING - CORYNEBACTERIUM DIPTHERIAE

ALBERT STAINING – PRINCIPLE, REQUIREMENTS, PROCEDURE & RESULT INTERPRETATION

INTRODUCTION TO ALBERT STAINING Corynebacterium is the genus of Gram +ve, non-acid-fast, non-sporing, non-motile bacilli. The most important member of the genus is Corynebacterium diphtheriae, the causative agent of Diphtheria disease in children. The C. diptheriae or diphtheria bacillus was first described by Klebs but Loffler was the first to cultivate it in the laboratory and also called as the Klebs-Loffler’s bacillus (KLB) They form metachromatic granules, these granules are ...
Continue Reading.....
bacterial smear preparation - bacterial smear - microscopic examination of specimen in microbiology - bacterial smear preparation in lab - how to prepare a bacterial smear

PREPARATION OF AN IDEAL BACTERIAL SMEAR

A bacterial smear is required particularly for the microscopic examination of the specimen or the culture for the identification of bacteria which is done by preparing the smear from the specimen or culture followed by staining with the appropriate stains, e.g. Gram staining. The purpose of making the smear is to fix the specimen or the bacterial cells from the culture onto the microscopic glass slide so that it will ...
Continue Reading.....
Loading...