HISTOLOGICAL TECHNIQUES

HISTOLOGICAL TECHNIQUES

HISTOLOGICAL TECHNIQUES

Histological techniques are the techniques which have been developed for the processing of the specimens, mainly tissues, for the proper diagnosis of the diseases associated. The small pieces of the tissues or sometimes whole organs are submitted to the histopathology laboratory for the diagnosis of any abnormalities if present. These tissues or organs need the treatment of certain chemicals to prevent any deterioration or alterations before the exact examination under the microscope is carried out. There are various techniques for the tissue processing as per the requirements of the tests prescribed or for the research purposes but the most commonly used is Paraffin method. This includes Fixation, Dehydration, Clearing, Impregnation, and Embedding.

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FIXATION OF TISSUES

FIXATION – THE TECHNIQUE OF FIXING THE TISSUES

FIXATION – THE TECHNIQUE OF FIXING THE TISSUES Fixation is a process by which the constituents of the cells and thereby tissues, are fixed in a physical and partly chemical state, so that they are able to withstand subsequent treatment with various chemicals, with minimum or no significant distortion or ...
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DEHYDRATION OF TISSUES

DEHYDRATION OF TISSUES – TECHNIQUE OF REMOVING WATER FROM THE TISSUES

DEHYDRATION OF TISSUES – TECHNIQUE OF REMOVING WATER FROM THE TISSUES Dehydration of tissues is the important process because of the paraffin, in which the tissues are embedded, is not miscible with water and does not penetrate the tissue effectively. Hence water in the tissue should be removed before embedding; this ...
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CLEARING OF TISSUES

CLEARING OF TISSUES – THE TECHNIQUE OF MAKING THE TISSUES TRANSPARENT

CLEARING OF TISSUES – THE TECHNIQUE OF MAKING THE TISSUES TRANSPARENT After dehydrating the tissues, the alcohol present in them must be removed before they are impregnated and embedded with paraffin. The Paraffin wax used for embedding is immiscible in both alcohol and water. Thus the alcohol must be replaced by some ...
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IMPREGNATION AND TISSUE EMBEDDING

IMPREGNATION AND TISSUE EMBEDDING

IMPREGNATION AND TISSUE EMBEDDING The tissues, after fixation and dehydration process, are not sufficiently hard to cut into thin sections without a suitable support. Thus, tissues are first impregnated and then embedded in a suitable embedding medium to make a block which makes the tissue quite hard and provides a suitable support so that it can ...
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