BIOCHEMICAL TESTS FOR STAPHYLOCOCCUS AUREUS
BIOCHEMICAL TESTS FOR STAPHYLOCOCCUS AUREUS - STAPH AUREUS BIOCHEMICAL TESTS - BIOCHEMICAL REACTIONS OF MICROORGANISMS

BIOCHEMICAL TESTS FOR STAPHYLOCOCCUS AUREUS

BIOCHEMICAL TESTS FOR STAPHYLOCOCCUS AUREUS

There are so many biochemical reactions for the Staphylococcus aureus but a few reactions are most commonly used and are medically important for distinguishing pathogenic staphylococcus i.e. S. aureus from other non- pathogenic Staphylococci which are as follows:

  • Beta hemolysis on Blood agar
  • Golden yellow pigment production
  • Coagulase production
  • Mannitol Fermentation
  • Gelatin liquefaction
  • Phosphatase production
  • Deoxyribonuclease (DNase) production
  • Tellurite reduction

Below is the list of various other biochemical tests for Staphylococcus aureus which have great importance in research:

TESTRESULT
Coagulase
+VE (POSITIVE)
Catalase+VE (POSITIVE)
Gelatin Hydrolysis+VE (POSITIVE)
H2S production-VE (NEGATIVE)
Gas production-VE (NEGATIVE)
Indole-VE (NEGATIVE)
MR (Methyl Red)+VE (POSITIVE)
VP (Voges Proskauer)+VE (POSITIVE)
Citrate+VE (POSITIVE)
PYR-VE (NEGATIVE)
Urease+VE (POSITIVE)
DNase+VE (POSITIVE)
Oxidase-VE (NEGATIVE)
Phosphatase+VE (POSITIVE)
Nitrate Reduction+VE (POSITIVE)
Hyalurodinase+VE (POSITIVE)
Lipase+VE (POSITIVE)

Staphylococcus aureus and other species of staphylococci ferment various sugars producing acid without gas but these sugar fermentation tests are of no diagnostic value in routine laboratory tests except Mannitol fermentation which is of great importance in differentiating Staphylococcus aureus which is mannitol positive from other non-pathogenic Staphylococci especially from Staphylococcus epidermidis. The other sugar fermentation tests are of great importance in research work.

SUGARSRESULTS
Glucose+VE (POSITIVE)
Fructose+VE (POSITIVE)
Lactose+VE (POSITIVE)
Galactose+VE (POSITIVE)
Maltose+VE (POSITIVE)
Arabinose-VE (NEGATIVE)
Mannitol+VE (POSITIVE)
Mannose+VE (POSITIVE)
Raffinose-VE (NEGATIVE)
Ribose+VE (POSITIVE)
Sucrose+VE (POSITIVE)
Xylose-VE (NEGATIVE)



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